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hygromycin  (Addgene inc)


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    Structured Review

    Addgene inc hygromycin
    Hygromycin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plat1+cas9/BB3cH_pGAP_23*_pLAT1_Cas9+(Plasmid+%23104907)/bio_rxiv__64898__2026__02__17__706399-216-16-17
    Average 93 stars, based on 3 article reviews
    hygromycin - by Bioz Stars, 2026-09
    93/100 stars

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    Clone Assay:

    Article Title: Engineering the synthetic β-alanine pathway in Pichia pastoris for conversion of methanol into 3-hydroxypropionic acid
    Article Snippet: Plasmids/Strains Modules/Genotype Reference Plasmids BB1_12_pAOX1 PAOX1, KanR+ [5] BB1_12_pFDH1 PFDH1, KanR+ BB1_12_pPOR1 PPOR1, KanR+ BB1_12_pPDC1 PPDC1, KanR+ BB1_23 Ø, KanR+ BB1_34_RPS3tt RPStt, KanR+ BB1_34_ScCYC1tt ScCYC1tt, KanR+ BB1_34_TDH3tt TDH3tt, KanR+ BB2_BC Ø, AmpR+ pCC1-4k_TcPAND panD+, T. castaneum gene encoding for PANDTc enzyme GenScript Biotech pCC1-4k_BcBAPAT yhxA+, B. cereus gene encoding for BAPATBc BB1_23_EcYDFG ydfG+, E. coli str. .. K-12 gene encoding for YDFGEc BB1_23_PseFDH(V9) fdh+, Pseudomonas sp. (strain 101) gene encoding for FDHPse(V9) (A199G/D222Q/S381V/C256A/H380K) BB2_BC_TcPAND PAOX1-PANDTc-RPS3tt This work BB2_BC_BcBAPAT PFDH1-BAPATBc-ScCYC1tt BB2_BC_EcYDFG PPOR1-YDFGEc-TDH3tt PPDC1-YDFGEc-TDH3tt BB2_BC_PseFDH(V9) PFDH1-FDH(V9)Pse-TDH3tt Page 14/30 Plasmids/Strains Modules/Genotype Reference Plasmids BB3nK_ext_AD Ø, KanR+ [6] BB3nK_ext_AD_TcPAND 5’-HR_PAOX1-PANDTc-RPS3tt_3’-HR(RGI2) This work 5’-HR_PAOX1-PANDTc-RPS3tt_3’-HR(pGAPUP) BB3nK_ext_AD_BcBAPAT 5’-HR_PFDH1-BAPATBc-ScCYC1tt_3’HR(pTEF1-αUP) BB3nK_ext_AD_EcYDFG 5’-HR_PPOR1-YDFGEc-TDH3tt_3’HR(pFLD1UP) 5’-HR_PPDC1-YDFGEc-TDH3tt_3’HR(pFLD1UP) BB3nK_ext_AD_PseFDH(V9) 5’-HR_PFDH1-FDH(V9)Pse-TDH3tt_3’HR(pAOX1UP) BB3cN_pGAP_23*_pLAT1_Cas9 PGAP_ Ø_PLAT1_Cas9, NrsR+ [6] BB3cK_pGAP_23*_pLAT1_Cas9 PGAP_ Ø_PLAT1_Cas9, KanR+ BB3cH_pGAP_23*_pLAT1_Cas9 PGAP_ Ø_PLAT1_Cas9, HygR+ BB3cK_pGAP_gRNA1(RGI2) _pLAT1_Cas9 PGAP_ gRNA1(RGI2)_PLAT1_Cas9, KanR+ This work BB3cH_pGAP_gRNA1(pGAPUP) _pLAT1_Cas9 PGAP_ gRNA1(PGAPUP)_PLAT1_Cas9, HygR+ BB3cK_pGAP_gRNA2(pTEF1αUP) _pLAT1_Cas9 PGAP_ gRNA2(PTEF1−αUP)_PLAT1_Cas9, KanR+ BB3cN_pGAP_gRNA1(pFLD1UP) _pLAT1_Cas9 PGAP_gRNA1(PFLD1UP)_PLAT1_Cas9, NrsR+ BB3cN_pGAP_gRNA1(pAOX1UP) _pLAT1_Cas9 PGAP_gRNA1(PAOX1UP)_PLAT1_Cas9, NrsR+ P. pastoris strains CBS7435 Wild-type CBS, Centraalbureau voor Schimmelcultures Page 15/30 Plasmids/Strains Modules/Genotype Reference Plasmids PpCβ10 PAOX1_PANDTc + PFDH1_BAPATBc + PPOR1_YDFGEc This work PpCβ20 PAOX1_PANDTc + PFDH1_BAPATBc + PPDC1_YDFGEc PpCβ11 2x(PAOX1_PANDTc) + PFDH1_BAPATBc + PPOR1_YDFGEc PpCβ21 2x(PAOX1_PANDTc) + PFDH1_BAPATBc + PPDC1_YDFGEc PpCβ20-P PpCβ20 + PFDH1_FDH(V9)Pse PpCβ21-P PpCβ21 + PFDH1_FDH(V9)Pse The modular cloning vectors used in this work are described in Prielhofer et al. [5], and are available at Addgene (Watertown, MA, USA) as GoldenPiCS kit. .. The CRISPR/Cas9-mediated homology-directed genome editing protocol performed in this study is described in Gassler et al. [6], and a ready-to-use plasmid kit named CRISPi can also be found at Addgene.



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